Research progress of China's small-size nano-silver particles in anti-influenza virus

       As everyone knows, nano-silver powder has good bactericidal and antiviral effects, and Chinese scientific researchers have also conducted in-depth research in this area in recent years and have made certain progress. Among them, Mr. Yin Jianjian of Dalian University School of Medicine is more representative of the anti-parainfluenza type 3 effect of nanosilver. The following research data and conclusions are all reproduced in the original text and corresponding papers.



 Research results of anti-parainfluenza type 3 virus effect and mechanism of nano-silver:



1. The toxic effect of nano silver on MDCK and the toxicity of PIV3. The large non-toxic mass concentration of nano silver powder on MDCK (TC0) is 25 mg/L [8], and the TCID50 of PIV3 on MDCK cells is 10-1.35 / mL. When the nano-silver concentration is greater than 25 mg/L, it starts to inhibit the growth of MDCK. The toxic effect of nano-silver powder on MDCK is mainly manifested in the increase of cell particles, uneven size, slow proliferation, and cell retraction, rounding, and cluster shedding and death. The toxic effect of nano-silver on cells decreases with the decrease of the concentration of nano-silver solution, and the cell survival rate increases accordingly.



2. MTT method detects the inhibitory effect of nano-silver on PIV3. The results show that the cell survival rate of nano-silver treatment group (direct inactivation group, preventive administration group, treatment administration group) is statistically different from the virus control group. Meaning (P <0.01). See Table 1. Table 1 Comparison of MTT detection results in different groups x ± s Tab 1 Comparison of MTT assay results in different groups MTT value Cell survival rate/% Direct inactivation group 0.8273 ±0.04 94.81 ±0.02 a Preventive drug group 0.8120 ±0.04 93.05±0.05 a Therapeutic administration group 0.7882 ±0.01 90.32±0.02 a Normal cell group 0.8727 ±0.04 —Virus control group 0.2470 ±0.05 25.50 ±0.06 Nano silver control group 0.8308 ± 0.03 95.21±0.04 F value 361. 542 332.327 P value <0.01 <0.01 a: P <0.01, compared with the virus control group.



3. Detecting the inhibitory effect of nano-silver on PIV3 by immunofluorescence method. After MDCK is layered on a 6-well culture plate with a cover glass, the nano-silver and PIV3 are treated differently, mounted, and observed under an inverted microscope. As shown in Figure 1, there was no specific yellow-green fluorescence in the normal MDCK group, and the cell morphology was normal; in the virus control group and the solvent control group (solvent and PIV3 mixed group), MDCK showed strong specific yellow-green fluorescence. Cell swelling and rounding, gap enlargement, cell death or shedding; intracellular specific fluorescence in the preventive medication group, direct inactivation group, and therapeutic medication group are rare, the cell morphology changes little, and the cells shrink slightly, but the morphology Basically normal.